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蔡秀純 副教授兼所長 個人簡介

I.實驗室主持人 (Shiow-Chwen Tsai)

 

姓名

蔡秀純

學歷

國立陽明大學生理學博士

職稱

副教授

研究興趣

運動訊息傳遞研究、運動生理學、運動內分泌學、老年醫學、濫用藥物研究

授課科目

人體解剖生理學、運動生理學、健康老化學、運動生理實驗技術、進階運動內分泌學

聯絡方式

02-28718288 ext. 5813

傳真

02-2875-3996

e-mail

sctsai6@gmail.com

sctsai@utaipei.edu.tw

專業團體

中國生理學會會員

臺灣調適科學會會員

中華民國內分泌學會會員

美國內分泌學會會員

美國生殖學會會員

專長領域

運動生理學、濫用藥物研究、老化疾病研究

 

II. 專業服務

Pharmatheutical Research (reviewer, SCI journal)

Chinese Journal of Physiology (reviewer, SCI journal)

大專體育學刊reviewer

中華民國大專院校體育總會運動科學與醫學委員會委員

體委會運科研究及發展獎勵之審查委員

國科會專題研究計畫審查委員

台灣調適科學學術研討會座長

國立臺北護理健康大學激勵教師研發創意教學計畫補助審查委員

臺北市立大學運動科學研究所學位論文口試委員

臺北市立大學休閒運動管理學系碩士班學位論文口試委員

輔仁大學公共衛生學系碩士班學位論文口試委員

臺北醫學大學公共衛生學系碩士班學位論文口試委員

 

IV. 實驗技術 (Lab techniques)

1.西方點墨法 (Western blot analysis):分析特定蛋白的表現,例如:Connexin 43, eNOS, StAR, Aquporin 7, MAPK, AMPK etc.

2.酵素免疫分析法 (ELISA):分析血液、組織或細胞中特定分子的濃度,例如:Interleukin 1, Interleukin 4, Interleukin 18, TNFa, Ghrelin, luteinizing hormone, and testosterone.

3.Zymography:分析matrix metalprotease (MMP) 活性

  Electrophoresis mobility shift assay (EMSA):分析蛋白與promoter 結合能力

  其他化學分析:

(a)能量代謝分析 (如:Glycogen content, Citrate synthase activity),

(b)氧化壓力及抗氧化能力分析 (MDA, TBARS, and activities of anti-oxidant enzymes, GSH content etc.)

4.組織染色 (Staining):Masson’s trichrome stain (膠原蛋白分佈), Hematoxylin and Eosin stain (病理組織染色), Immunofluorescence stain (免疫螢光染色), Immunohistochemistry stain(免疫組織染色).

5. 細胞培養 (Cell culture)

(a)初代培養 (primary cells):內皮細胞 (endothelial cells), 血管平滑肌細胞,心肌細胞,睪丸萊氏細胞 (Leydig cells of testis), 卵巢顆粒細胞 (Granulosa cells of ovarian follicles), 腎上腺皮質細胞 (Zona glomerulosa and reticularis cell of adrenal gland), 肝臟星狀細胞 (Hepatic Stellate Cell)

(b)細胞株:前列腺癌細胞 (prostate cancer cell line), 腎上腺皮質細胞株 (H295 cells)

6. 動物手術 (Animal Surgery)

(a)留置導管 (Catheterization) 以利動物於清醒無壓力下進行給藥及採集血樣

(b)尾靜脈採血、心室採血、組織或器官摘除

(c)腦下腺前葉移植 (Anterior pituitary graft)誘發高泌乳素血症 (hyperprolactinemia)

(d)5/6 腎臟切除

V. 研究計畫 (Research projects)

 

           

擔任工作

計畫支援機關

起迄年月

安非他命抑制雌鼠卵巢顆粒細胞助孕酮分泌之機制-抑制StAR protein 表現與造成細胞凋亡機制的探討

主持人

行政院衛生署管制藥品管理局

2003/01/01~2003/12/31

糖皮質激素缺乏影響睪丸萊氏細胞睪固酮分泌之分子機制探討

主持人

財團法人新光吳火獅紀念醫院

2004/01/01~2004/12/31

安非他命造成心肌病變之細胞內分子機制探討

主持人

行政院國家科學委員會

2004/08/01~2005/07/31

吳茱萸萃取物抑制腫瘤侵襲的分子機制研究

主持人

財團法人新光吳火獅紀念醫院

2005/01/01~2005/12/31

安非他命誘發心肌細胞凋亡的機制探討

主持人

行政院國家科學委員會

2005/08/01~2006/07/31

氧化自由基抑制萊氏細胞StAR蛋白表現之分子機轉研究

主持人

財團法人新光吳火獅紀念醫院

2006/01/01~2006/12/31

甲基安非他命抑制卵巢濾泡成熟之機制探討

主持人

行政院衛生署

管制藥品管理局

2006/01/01~2006/12/31

安非他命透過Rho Kinase Family 誘發心肌肥大

主持人

行政院國家科學委員會

2006/08/01~2007/07/31

愷他命抑制卵巢濾泡細胞MAPK磷酸化及濾泡發育

主持人

財團法人新光吳火獅紀念醫院

2007/01/01~2007/12/31

甲基安非他命透過脂質過氧化及活化 MAPK蛋白造成睪丸細胞損傷

主持人

財團法人新光吳火獅紀念醫院

2008/01/01~2008/12/31

補充精胺酸降低衰竭運動誘發心肌損傷之細胞內分子機制研究

共同主持人

行政院國家科學委員會

2008/08/01~2009/07/31

補充精胺酸降低衰竭運動誘發骨骼肌凋亡之細胞分子機制研究

共同主持人

行政院國家科學委員會

2008/08/01~2009/07/31

補充精胺酸改善胰島素阻抗之機制探討

共同主持人

行政院國家科學委員會

2009/08/01~2010/07/31

精胺酸降低衰竭運動誘發心肌凋亡之機制探討

主持人

行政院國家科學委員會

2010/08/01~2011/07/31

補充精胺酸降低衰竭運動誘發心肌纖維化的分子機制

主持人

行政院國家科學委員會

2011/08/01~2012/07/31

發育中棒球選手身體構造特徵及運動表現之相關性研究

共同主持人

行政院國家科學委員會

2011/08/01~2013/07/31

 

 

VI.學術發表

 

A. Original articles

  1. Wang PS, Tsai· SC, Hwang GS, Wang SW, Lu CC, Chen JJ, Liu SR, Lee KY, Chien EJ, Chien CH. Calcitonin inhibits testosterone and luteinizing hormone secretion through a mechanism involving an increase in cAMP production in rats. Journal of Bone and Mineral Research 1994; 9(10):1583-1590.
  2. Tsai SC, Chiao YC, Lu CC, Doong M, Chen YH, Shih HC, Liaw C, Wang S, Wang PS. Inhibition by amphetamine of testosterone secretion through a mechanism involving an increase of cyclic AMP production in rat testes. British Journal of Pharmacology 1996; 118(4): 984–988.
  3. Lu SS, Lau CP, Tung YF, Huang SW, Chen YH, Shih HC, Tsai SC, Lu CC, Wang SW, Chen JJ, Chien EJ, Chien CH, Wang PS. Lactate stimulates progesterone secretion via an increase in cAMP production in female rats. The American journal of physiology 1996; 271(5 Pt 1):E910-915.
  4. Tsai SC, Chen JJ, Chiao YC, Lu CC, Lin H, Yeh JY, Lo MJ, Kau MM, Wang SW, Wang PS. The role of cyclic AMP production, calcium channel activation and enzyme activities in the inhibition of testosterone secretion by amphetamine. British Journal of Pharmacology 1997; 122(5): 949–955.
  5. Chen TS,·Doong ML, Wang SW, Tsai SC, Lu CC, Shih HC, Chen YH, Chang FY, Lee SD, Wang PS. Gastric emptying and gastrointestinal transit during lactation in rat. The American Journal of Physiology 1997; 272(3 Pt 1):G626-631.
  6. Lu SS, Lau CP, Tung YF, Huang SW, Chen YH, Shih HC, Tsai SC, Lu CC, Wang SW, Chen JJ, Chien EJ, Chien CH, Wang PS. Lactate and the effects of exercise on testosterone secretion: evidence for the involvement of a cAMP-mediated mechan Tsai SC, Chen CW, Yu PL, and Wang PS. Adaptation and dysadaptation of abuse-Independent physiological responses to amphetamine. Adaptive Medicine 2014; 6 (4): 137-147.ism. Medicine and Science in Sports and Exercise 1997, 29(8):1048-1054.
  7. Doong ML, Lu CC, Kau MM, Tsai SC, Chiao YC Chiao, Chen JJ, Yeh JY, Lin H, Huang SW, Chen TS,  Chang FY, Wang PS. Inhibition of gastric emptying and intestinal transit by amphetamine through a mechanism involving an increased secretion of CCK inmale rats. British Journal of Pharmacology 1999; 124: 11123-1130.
  8. Huang WJ, Yeh JY, Tsai SC, Lin H, Chiao YC, Chen JJ, Lu CC, Hwang SW, Wang SW, Chang LS, Wang PS. Regulation of testosterone secretion by prolactin in male rats. Journal of Cellular Biochemistry 1999; 74(1):111-118.
  9. Kau MM, Lo MJ, Tsai SC, Chen JJ, Pu HF, Chien EJ, Chang LL, Wang PS. Effects of prolactin on aldosterone secretion in rat zona glomerulosa cells. Journal of Cellular Biochemistry 1999; 72(2):286-293.
  10. Kau MM, Lo MJ, Tsai SC, Chen JJ, Lu CC, Lin H, Wang SW, Wang PS. Effects of estradiol on aldosterone secretion in ovariectomized rats. Journal of Cellular Biochemistry 1999; 73(1):137-144.
  11. Lin H, Wang SW, Tsai SC, Chen JJ, Chiao YC, Lu CC, Huang WJS, Wang GJ, Chen CF, Wang PS. Inhibitory effect of digoxin on testosterone secretion through mechanisms involving decrease of cyclic AMP production and cytochrom P450scc activity in rat testicular interstitial cells. British Journal of Pharmacology 1999; 125(8):1635-1640.
  12. Lu CC, Tsai SC, Huang WJS, Tsai CL, Wang PS. Effects of hyperprolactinemia on calcitonin secretion in male rats. Metabolism 1999; 48(2):221-226.
  13. Tsai SC, Lu CC, Chen JJ, Chiao, YC, Wang SW, Hwang, Wang PS. Inhibition of salmon calcitonin on secretion of progesterone and GnRH-stimulated pituitary luteinizing hormone. The American Journal of Physiology 1999; 277(1 Pt 1):E49-55.
  14. Yeh JY, Tsai SC, Kau MM, Lo MJ, Wang PS. Effects of thyroid hormones on the release of Calcitonin Gene-Related Peptide (CGRP) by rat prostate glands in vitro. The Chinese Journal of Physiology 1999; 42(2):89-94.
  15. Lin H, Tsai SC, Chen JJ, Chiao YC, Wang SW, Wang GJ, Chen CF, Wang PS. Effect of evodiamine on the secretion of testosterone in rat testicular interstitial cells. Metabolism 2000; 48(12-48):1532-1535.
  16. Lu CC, Tsai SC, Chien EJ, Tsai CL, Wang PS. Age-related differences in the secretion of calcitonin in male rats. Metabolism 2000; 49(2):253-258.
  17. Wang R, Tsai SC, Chen JJ, Wang PS. The simulation effects of mountain climbing training on selected endocrine responses. The Chinese Journal of Physiology 2001; 44(1):13-18
  18. Tsai SC, Chiao YC, Lu CC, Wang PS. Stimulation of the secretion of luteinizing hormone by ginsenoside-Rb1 in male rats. The Chinese Journal of Physiology 2003; 46(1):1-7.
  19. Tsai SC, Lu CC, Lin CS, Wang PS. Antisteroidogenic Actions of Hydrogen Peroxide on Rat Leydig Cells. Journal of Cellular Biochemistry 2003; 90(6):1276-1286.
  20. Lin SK, Tsai SC, Lee CC, Wang BW, Liou JY, Shyu KG. Berberine inhibits HIF-1α expression via enhanced proteolysis. Molecular Pharmacology 2004; 66(3):612-619
  21. Shyu KG, Tsai SC, Wang BW, Liu YC, Lee CC. Saikosaponin C induces endothelial cells growth, migration and capillary tube formation. Life Science 2005; 76(7):813-26.
  22. Lee CC, Chen SC, Tsai SC, Wang BW, Liu YC, Lee HM, Shyu KG. Hyperbaric oxygen induces VEGF expression through ERK, JNK and c-Jun/AP-1 activation in human umbilical vein endothelial cells. Journal of Biomedical Science 2006; 13(1):143-156.
  23. Lin WT, Yang SC, Tsai SC, Huang CC, and Lee NY. L-arginine attenuated xanthine oxidase and myeloperoxidase activities in hearts of rats during exhaustive exercise. British Journal of Nutrition 2006; 95:67-75. (SCI)
  24. Shyu KG, Lin S, Lee CC, Chen Eden, Lin LC, Wang BW, and Tsai SC. Evodiamine inhibits in vitro angiogenesis: Implication for antitumorgenicity. Life Science 2006; 78: 2234-2243. (SCI)
  25. Tsai SC, Liu YC, Li CP, Huang TS, and Lee CC. Sesamin inhibits vascular endothelial cell growth and angiogenic activity of lung adenocarcinoma cells. Journal of Cancer Molecule 2006; 2:199-205.
  26. Lu CC, and Tsai SC. The cyclic AMP-dependent protein kinase A pathway is involved in progesterone effects on calcitonin secretion from TT cells. Life Science 2007; 81:1411-1420.
  27. Huang CC, Tsai SC, and Lin WT. Potential ergogenic effects of l-arginine against oxidative and inflammatory stress induced by acute exercise in aging rats. Experimental Gerontology. 2008; 43:571-577.
  28. Chen MC, Wang SW, Kan SF, Tsai SC, Wu YC, and Wang PS. Stimulatory Effects of Propylthiouracil on Pregnenolone Production through Upregulation of Steroidogenic Acute Regulatory Protein Expression in Rat Granulosa Cells. Toxicological Science 2010; 118:667-674.
  29. Chou CC, Bai CH, Tsai SC, and Wu MS. Low Serum acylated ghrelin levels are associated with the development of cardiovascular disease in hemodialysis patients. Internal Medicine 2010; 49:2057-2064.
  30. Tsai SC, Chen CP, Su TH, Kau MM, and Lu CC. Involvement of ERK phosphorylation in the prevention of ischemia-induced ovarian follicular depletion by stem cells. Chin. J. Physiol. 2010; 53:167-177.
  31. Chan CC, Tsai SC, Cheng LY, Lee FY, Lin HC. Hemodynamic assessment of the development of portal-systemic collaterals in portal hypertensive rats. Digestive Diseases and Sciences 2011; 56: 417-424.
  32. Lu CC, and Tsai SC. Involvement of the cyclic-AMP-dependent protein kinase A pathway in thyroxine effects on calcitonin secretion from TT cells. Hormone Metabolic Research 2011; 43: 31-36.
  33. Wong SL, Chang HS, Wang GJ, Chiang MY, Huang HY, Chen CH, Tsai SC, Lin CH, Chen IS. Secondary metabolites from the roots of Neolitsea daibuensis and their anti-inflammatory activity. Journal of Natural Products 2011;74(12):2489-2496. Epub 2011 Dec 7.
  34. Kau MM, Wang JR, Tsai SC, Yu CH, Wang PS. Inhibitory effect of bufalin and cinobufagin on steroidogenesis via the activation of ERK in human adrenocortical cells. British Journal of Pharmacology 2012;165(6):1868–1876.
  35. Lin SJ, Tu YK, Tsai SC, Lu HK. Nonsurgical periodontal therapy with and without subgingival minocycline administration in patients with poorly controlled type II diabetes. A randomized controlled clinical trial. Clinical Oral Investigation, 2012; 16:599–609.
  36. 2013Tsai YL, Tsai SC, Yen SH, Huang KL, Mu PF, Liou HC, Wong TT, Lai IC, Liu P, Lou HL, Chiang IT, and Chen YW. Efficacy of therapeutic play for pediatric brain tumor patients during external beam radiotherapy. Child's Nervous System, 2013; 29(7):1123-1129.
  37. Tsai SC, Chen CW, Yu PL, and Wang PS. Adaptation and dysadaptation of abuse-Independent physiological responses to amphetamine. Adaptive Medicine 2014; 6 (4): 137-147.
  38. Lin JF, Lin YH, Liao PC, Lin YC, Tsai TF, Chou KY, Chen HE, Tsai SC, Hwang TIS. Induction of testicular damage by daily methamphetamine administration in rats. The Chinese Journal of Physiology 2014; 57(1):19-30.
  39. Liou CM, Tsai SC, Kuo CH, Ting H,  Lee SD. Cardiac Fas-dependent and mitochondria-dependent apoptosis after chronic cocaine abuse. Internal Journal of Molecular Science, 2014;15(4), 5988-6001.
  40. Liou, C. M., Tsai, S. C., Kuo, C. H., Williams, T., Ting, H., & Lee, S. D. Chronic methamphetamine exposure induces cardiac fas-dependent and mitochondria-dependent apoptosis. Cardiovascular Toxicology, 2014;14(2), 134-144.

 

B. Conference articles

  1. Cheng HY, Tsai SC, Huang WC, Yang AL., and Chan CC  Effects of coffee consumption on NASH-induced oxidative stress in rat heart.  (Abstract# S271) 2012 Experimental Biology, Apr 21-25, 2012, San Diego Convention Center, San Diego, CA, USA
  2. Huang WC, Chan CC, Hung SK, Yang AL, and Tsai SC.    Effects of exercise training on NASH-induced oxidative stress in heart of rats (Abstract #S321)   2012 Experimental Biology, Apr 21-25, 2012, San Diego Convention Center, San Diego, CA, USA
  3. Hung SK, Chan CC, Huang WC, and Tsai SC     Exercise training increased SOD activity in liver of NASH rats (Abstract #2135)      ACSM 59th Annual Meeting, May 30-Jun 2, 2012, San Fransico, CA, USA
  4. Tsai SC, Chiu KH, and Chang CJ. The mechanisms of experimental retractile testicle induced testicular injury(S-25)     The Second Cross-Strait Symposium on Adaptive Medicine and Adaptability, Jinan, Shandong, China. 14-16 Oct, 2011.
  5. Huang WC, Chen CC, and Tsai SC.      Mechanisms of methamphetamine on the activity of antioxidant system in rat liver (S-13)     Symposium of Metabolic Adaptation to Exercise Physiology, Taipei, Taiwan, ROC. 9-10 Apr, 2011.
  6. Hung SK, Chen CC, and Tsai SC. Mechanisms of the inhibition of cocaine on the expression of antioxidant enzymes in rat liver (S-14)      Symposium of Metabolic Adaptation to Exercise Physiology, Taipei, Taiwan, ROC. 9-10 Apr, 2011.
  7. Chiu KH, Chang CJ, and Tsai SC. Effects of cryptorchidism on testicular function in rats.        Symposium of Metabolic Adaptation to Exercise Physiology, Taipei, Taiwan, ROC. 9-10 Apr, 2011.
  8. Hsu CM, Chen CC, and Tsai SC.   The protective effect of L-arginine against exhaustive exercise-induced oxidative stress in kidney (S-24)   Symposium of Metabolic Adaptation to Exercise Physiology, Taipei, Taiwan, ROC. 9-10 Apr, 2011.
  9. Hsiao YH, and Tsai SC. The risk factor of metabolic syndrome and severity of epilepsy (S-25) Symposium of Metabolic Adaptation to Exercise Physiology, Taipei, Taiwan, ROC. 9-10 Apr, 2011.
  10. Bai CH, Chen HS, Liu WC, and Tsai SC.      Predictive efficiency of physical activity for cognitive change in community-based elderly.       Symposium of Metabolic Adaptation to Exercise Physiology, Taipei, Taiwan, ROC. 9-10 Apr, 2011.
  11. 張秀娟,陳智傑,l廖辰中,蔡秀純   衰竭運動對大鼠血清白蛋白修飾之影響    2011運動教練科學學術研討會, July 9-10, 2011, Taipei, Taiwan, ROC
  12. 張秀娟,陳智傑,l廖辰中,蔡秀純   精胺酸及衰竭運動對大鼠血清白蛋白修飾之交互影響    2011運動教練科學學術研討會, July 9-10, 2011, Taipei, Taiwan, ROC
  13. Kau MM, Wang JR, Tsai SC, Huang SK, and Wang PS.      Effect of food restriction on the steroidogenesis of aldosterone in ovariectomized rats (S-30)      The First Symposium on Adaptive Medicine and Adaptability across the Strait, Taipei, Taiwan, ROC. 11-14 Sep, 2010.
  14. Chen CC, and Tsai SC    Mechanism of methamphetamine affects activities of antioxidant system in rat liver.        The First Symposium on Adaptive Medicine and Adaptability across the Strait, Taipei, Taiwan, ROC. 11-14 Sep, 2010.
  15. Tsai SC, and Lu CC. Stem cells prevent radiation exposure-induced ovarian follicular depletion. (Poster Session C, Program# 699) Program and Abstract of the 43th Annual Meeting of the Society for the Study of Reproduction, Midwest Airlines Center, Milwaukee, Wisconsin, 30 July-3 Aug, 2010.
  16. Lu CC, and Tsai SC. Effect of PGE2 on TT cells viability and division. (Poster Session B, Program# 422) Program and Abstract of the 43th Annual Meeting of the Society for the Study of Reproduction, Midwest Airlines Center, Milwaukee, Wisconsin, 30 July-3 Aug, 2010.
  17. Hsu CM, Chang HC, Chen CC, Tsai SC. Codonopsis pilosula extract reverse glucose tolerance impairment-induced sperm dysfunction. (Poster Session B, Program# 518) Program and Abstract of the 43th Annual Meeting of the Society for the Study of Reproduction, Midwest Airlines Center, Milwaukee, Wisconsin, 30 July-3 Aug, 2010.
  18. Chiu KH, Chang HC, Chen CC, Tsai SC. Antioxidant Effects of Codonopsis pilosula reverse glucose tolerance impairment-induced testicular dysfunction (Poster Session C, Program# 519) Program and Abstract of the 43th Annual Meeting of the Society for the Study of Reproduction, Midwest Airlines Center, Milwaukee, Wisconsin, 30 July-3 Aug, 2010.
  19. Tsai SC, and Lu CC. Amphetamine inhibits progesterone release via altered StAR-related signal transduction. (Abstract# P2PM-6-4) 36th International Congress of Physiological Sciences, July 27-Aug 1, 2009, Kyoto, Japan.
  20. Chen CC, Tsai SC, and Lu CC. Methamphetamine inhibits progesterone release through increasing connexin 43 expression and ERK phosphorylation in granulose cells. (Abstract# P2PM-6-5) 36th International Congress of Physiological Sciences, July 27-Aug 1, 2009, Kyoto, Japan.
  21. Liu WC, Chiang HS, and Tsai SC. Administration of methamphetamine inhibits maturation of ovarian follicles. (Abstract# P3PM-4-8) 36th International Congress of Physiological Sciences, July 27-Aug 1, 2009, Kyoto, Japan.
  22. Cheng HS, Liu WC, and Tsai SC. Administration of methamphetamine inhibits folliculogenesis via inhibiting steroidogenic proteins. (Abstract# P3PM-4-9) 36th International Congress of Physiological Sciences, July 27-Aug 1, 2009, Kyoto, Japan.
  23. Lu CC, and Tsai SC. The Mechanism of PRL effects on CT secretion in TT cells, related with JAK2 and STAT5. (Abstract# P2PM-4-7) 36th International Congress of Physiological Sciences, July 27-Aug 1, 2009, Kyoto, Japan.
  24. Liu WC, Cheng HS, Bai CH, and Tsai SC. Fallers and cognitive function decline: The study among the community-dwelling elders. (Abstract# P58) IX World Congress of the International Society for Adaptive Medicine, Agu 2-Aug 5, 2009, Taipei, Taiwan.
  25. Cheng HS, Liu WC, Tsai SC, and Bai CH. Physical activity status with aging in community-dwelling elder. (Abstract# P59) IX World Congress of the International Society for Adaptive Medicine, Agu 2-Aug 5, 2009, Taipei, Taiwan.
  26. Tsai SC, and Lu CC. Mechanisms of Methamphetamine Inhibited Folliculogenesis in Ovary. (Invited speaker, IX World Congress of the International Society for Adaptative Medicine, Symposium 15, S-80, August 2-5, 2009, Taipei, Taiwan)
  27. Chen CC, and Tsai SC. Methamphetamine induced liver remodeling via inhibited activities of anti-oxidant enzymes (Abstract No. PE464, 19th Conference of the APASL, February 13-16, 2009, Hong Kong).

 

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